Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Standardized Extract (HemoHIM) Protects against Scopolamine-Induced Amnesia in a Murine Model

doi: 10.1155/2021/8884243

Figure Lengend Snippet: The effects of HemoHIM on (a) Ach concentration and (b) AchE activity in the hippocampus. Ach levels ( n = five per group) and AchE ( n = 6 per group) in the hippocampus were determined using the ELISA kit. The data are expressed as the mean ± S.E.M. Significant difference from control group (### P < 0.001) and from scopolamine-treated group ( ∗∗∗ P < 0.001, ∗ P < 0.05).

Article Snippet: Acetylcholine ELISA kit and acetylcholinesterase ELISA kit were purchased from Elabscience Biotechnology Co. (Houston, TX, USA).

Techniques: Concentration Assay, Activity Assay, Enzyme-linked Immunosorbent Assay, Control

Journal: MedComm

Article Title: Mas‐Related G Protein‐Coupled Receptor Member D Sustains Hypertension

doi: 10.1002/mco2.70706

Figure Lengend Snippet: Effect of MrgD on blood pressure. (A) Flow chart of Ad‐MrgD‐treated SD rats; (B–D) the SBP, DBP, and MAP of Ad‐MrgD‐treated SD rats, n = 8 biological replicates per group. (E) Flow chart of MrgD shRNA‐treated SHR rats; (F–H) the SBP, DBP, and MAP of MrgD shRNA‐treated SHR rats, n = 8 biological replicates per group. (I) Flow chart of Ang II‐treated MrgD KO mice; (J–L) the SBP, DBP, and MAP of Ang II‐treated MrgD KO mice, n = 8 biological replicates per group. (M) Representative images of endothelial‐dependent (PFG2α and Ach) vasodilation and vasoconstriction responses. (N) Contraction effect curve of PGF2α‐induced mesenteric arteries, n = 6 biological replicates per group; (O) diastolic effect curve of Ach‐induced mesenteric arteries, n = 6 biological replicates per group. (P) Representative images of non‐endothelial‐dependent (NE and SNP) vasodilation and vasoconstriction responses. (Q) Contraction effect curve of NE‐induced mesenteric arteries, n = 6 biological replicates per group; (R) diastolic effect curve of SNP‐induced mesenteric arteries, n = 6 biological replicates per group. The data were expressed as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Subsequently, vasoconstrictive responses were elicited by cumulative concentrations of PGF2α (Sigma, CAS 4510‐16‐1) or NE (MCE, HY‐13715) over a concentration range of 10 −9 to 10 −4 M. Endothelium‐dependent and ‐independent vasorelaxation were assessed with Ach (MCE, HY‐B0282) and SNP (MCE, HY‐B0564), respectively, using the same concentration range (10 −9 to 10 −4 M).

Techniques: shRNA

Journal: MedComm

Article Title: Mas‐Related G Protein‐Coupled Receptor Member D Sustains Hypertension

doi: 10.1002/mco2.70706

Figure Lengend Snippet: Risperidone alleviated hypertension and remodeling of the mesenteric artery. (A) The structure of risperidone; (B) the structure of risperidone binding with MrgD; (C–E) risperidone relieved Ang II‐induced hypertension in mice, * vs. Saline and # vs. Ang II, n = 8 biological replicates per group. (F and G) Risperidone reversed Ang II‐induced increases in the mRNA levels of collagen I and TGF‐β in the mesenteric artery of mice, n = 5 biological replicates per group. (H–K) Risperidone reversed Ang II‐induced changes in the protein levels of collagen I, TGF‐β, and SM‐22α in the mesenteric artery of mice, n = 5 biological replicates per group. (L) Representative images of endothelial‐dependent (PFG2α and Ach) and non‐endothelial‐dependent (NE and SNP) vasodilation and vasoconstriction responses; (M and N) risperidone reduced vasorelaxation in the mesenteric artery of mice treated with Ang II, * vs. Saline and # vs. Ang II, n = 6 biological replicates per group. (O and P) Risperidone reduced contraction in the mesenteric artery of mice treated with Ang II, * vs. Saline and # vs. Ang II, n = 6 biological replicates per group. The data were expressed as mean ± SEM. */# p < 0.05, **/## p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Subsequently, vasoconstrictive responses were elicited by cumulative concentrations of PGF2α (Sigma, CAS 4510‐16‐1) or NE (MCE, HY‐13715) over a concentration range of 10 −9 to 10 −4 M. Endothelium‐dependent and ‐independent vasorelaxation were assessed with Ach (MCE, HY‐B0282) and SNP (MCE, HY‐B0564), respectively, using the same concentration range (10 −9 to 10 −4 M).

Techniques: Binding Assay, Saline